Journal: bioRxiv
Article Title: Tailoring a glycerol-free cryopreservation protocol with anti-freeze (glycol)proteins for commercial and native breeds of chicken
doi: 10.1101/2025.07.18.664799
Figure Lengend Snippet: Identification of very early (oviductal) embryo death. Germinal discs were gently removed from the vitelline membrane, immersed in 0.96 % NaCl and stained on microscope slides with propidium iodide. The presence of cell nuclei is an indicator of fertility. Unfertile germinal disc (left) and fertile germinal disc (right). (Photographs taken by Dr. Krisztina Liptói, NBGK)
Article Snippet: Motility was assayed using a computer-aided sperm analyzer system consisting on a camera (BASLER avA1000-100gc, Germany[WUR] / BASLER ace U acA1300-200uc, Germany [NBGK]) coupled to a phase contrast microscope (Zeis Axioscope A.1, Germany [WUR] / Nikon Eclipse E200, Japan [NBGK]), and using the 12500/0000 Androvision ® software (Minitübe, Germany) [WUR] / Sperm Class Analyzer (SCA; Barcelona, Spain) v.6.0.
Techniques: Membrane, Staining, Microscopy